Personal Narrative: I am Blind Essay -- essays research papers

I am blind. But I was not always blind. I once experienced the majesty of a sunrise in late August and the awe of the deep blue sea as the sun glistens from it. Now I have only my memories to comfort me. So they sent me here, a field trip, a good experience they say, to help me cope. They sent me to a deaf school for the day, unknowing of how awkward it is for me. They gave me a buddy, but for what? We can?t communicate through speech or sign language. ? You?ll figure it out,? they say. So now I sit here, alone and desolate in darkness, hoping for the occasional memory to write upon the blank screen of my vision and bring life to this seemingly dead place. And so I sit. The chair is hard and cold as stone, like a cement chair in a jail cell. It is firm, but not strong, it squeaks and wobbles around like a fish out of water. I reach down to make sure all four legs are there. Yes, there?s four, four cold smooth spears symbolizing my demise. Spears ready to leap forward and devour me if I make the slightest wrong or sudden move. I stretch out my arms, trying to find my surroundings. To the left, nothing but air. To the right, another spear, but not like the others this spears lies on its side like the rails in my bathroom. My hand follows the cold rod. It hits something leaving a sting and cracking my thumb as it crushes against my hand. This must be the desk. I glide my hand along the smooth surface felling all the little bumps of eraser left behind. I feel along the sides and touch the rigid edges where pens have been dug in deep like sharp wounds giving the desk a unique personality. As my fingertips reach the center, I re alize just how close the desk is, only about five inches away from my rapidly beatin... ... down the hall and I encounter a revelation of thought. We are going outside. I run faster now and burst through the front doors into the great heaven. I can smell fresh cut grass on the horizon and the soft warm rays of the sun finally beam down on my face. I breath the fresh cool air and listen. My bird has come back again and sings over the crackling flames and sirens of the trucks. It sings because it is free, it sings a victory praise for me because now I am free. I sit down on the wet grass and what I understand to be my buddy takes my hand. On my hand she spells out two letters. Two letters that say everything. She spells out ?O? ?K? and I cry because I know that no matter what happens or how much I have to suffer, in the end it?s okay because I am alive and I am free. So I am still blind, but now I am blind and loving it!

Affirmative Action Essay -- essays papers

Affirmative Action Few social policy issues have served as a better gauge of racial and ethnic divisions among the American people than affirmative action. Affirmative action is a term referring to laws and social policies intended to alleviate discrimination that limits opportunities for a variety of groups in various social institutions. Supporters and opponents of affirmative action are passionate about their beliefs, and attack the opposing viewpoints relentlessly. Advocates believe it overcomes discrimination, gives qualified minorities a chance to compete on equal footing with whites, and provides them with the same opportunities. Opponents charge that affirmative action places unskilled minorities in positions they are not qualified for and violates the Fourteenth Amendment. Since its inception, the definition of affirmative action has been ever-changing. Prohibiting discrimination in hiring, expanding the applicant pool to include more minorities, compensating for past grievances, and s etting quotas have all been part of the definition. In theory affirmative action helps integrate minorities better into society and puts them on equal footing with whites; however, in reality affirmative action is widening the racial gap in America and therefore should be discontinued. When the Civil Rights Law passed, minorities, especially African-Americans, believed that they should receive retribution for the years of discrimination that they endured. The government responded by passing laws to aide them in attaining better employment as reprieve for the previous two hundred years of suffering. To many, these laws made sense. After all, the white race was partly responsible for their enslavement. However, the individual w... ... Am I being picked for my writing ability, or to fulfill a quota† (Chinea-Varela 342). Minorities like Migdia want to be treated as equals, not incompetents. Affirmative Action was an essential part of the desegregation that occurred thirty years ago, but it has become outdated and is no longer part of the solution. It was a temporary plan to improve the conditions for minorities, but it is now more of a hindrance than a help to them. Affirmative action lowers standards, causes unqualified workers to be hired, places a stigma on minorities, lowers their confidence, and gives them the opportunity and encouragement to idle. Affirmative action has not fulfilled its goal of assisting lower income minorities with a history of discrimination, but instead has been exploited by middle-class minorities, the lower income groups still remaining uneducated and unsuccessful.

Chapter 1 The Riddle House

The villagers of Little Hangleton still called it â€Å"the Riddle House,† even though it had been many years since the Riddle family had lived there. It stood on a hill overlooking the village, some of its windows boarded, tiles missing from its roof, and ivy spreading unchecked over its face. Once a fine-looking manor, and easily the largest and grandest building for miles around, the Riddle House was now damp, derelict, and unoccupied. The Little Hangletons all agreed that the old house was â€Å"creepy.† Half a century ago, something strange and horrible had happened there, something that the older inhabitants of the village still liked to discuss when topics for gossip were scarce. The story had been picked over so many times, and had been embroidered in so many places, that nobody was quite sure what the truth was anymore. Every version of the tale, however, started in the same place: Fifty years before, at daybreak on a fine summer's morning when the Riddle House had still been well kept and impressive, a maid had entered the drawing room to find all three Riddles dead. The maid had run screaming down the hill into the village and roused as many people as she could. â€Å"Lying there with their eyes wide open! Cold as ice! Still in their dinner things!† The police were summoned, and the whole of Little Hangleton had seethed with shocked curiosity and ill-disguised excitement. Nobody wasted their breath pretending to feel very sad about the Riddles, for they had been most unpopular. Elderly Mr. and Mrs. Riddle had been rich, snobbish, and rude, and their grown-up son, Tom, had been, if anything, worse. All the villagers cared about was the identity of their murderer – for plainly, three apparently healthy people did not all drop dead of natural causes on the same night. The Hanged Man, the village pub, did a roaring trade that night; the whole village seemed to have turned out to discuss the murders. They were rewarded for leaving their firesides when the Riddles' cook arrived dramatically in their midst and announced to the suddenly silent pub that a man called Frank Bryce had just been arrested. â€Å"Frank!† cried several people. â€Å"Never!† Frank Bryce was the Riddles' gardener. He lived alone in a run-down cottage on the grounds of the Riddle House. Frank had come back from the war with a very stiff leg and a great dislike of crowds and loud noises, and had been working for the Riddles ever since. There was a rush to buy the cook drinks and hear more details. â€Å"Always thought he was odd,† she told the eagerly listening villagers, after her fourth sherry. â€Å"Unfriendly, like. I'm sure if I've offered him a cuppa once, I've offered it a hundred times. Never wanted to mix, he didn't.† â€Å"Ah, now,† said a woman at the bar, â€Å"he had a hard war, Frank. He likes the quiet life. That's no reason to -â€Å" â€Å"Who else had a key to the back door, then?† barked the cook. â€Å"There's been a spare key hanging in the gardener's cottage far back as I can remember! Nobody forced the door last night! No broken windows! All Frank had to do was creep up to the big house while we was all sleeping†¦Ã¢â‚¬  The villagers exchanged dark looks. â€Å"I always thought that he had a nasty look about him, right enough,† grunted a man at the bar. â€Å"War turned him funny, if you ask me,† said the landlord. â€Å"Told you I wouldn't like to get on the wrong side of Frank, didn't I, Dot?† said an excited woman in the corner. â€Å"Horrible temper,† said Dot, nodding fervently. â€Å"I remember, when he was a kid†¦Ã¢â‚¬  By the following morning, hardly anyone in Little Hangleton doubted that Frank Bryce had killed the Riddles. But over in the neighboring town of Great Hangleton, in the dark and dingy police station, Frank was stubbornly repeating, again and again, that he was innocent, and that the only person he had seen near the house on the day of the Riddles' deaths had been a teenage boy, a stranger, dark-haired and pale. Nobody else in the village had seen any such boy, and the police were quite sure Frank had invented him. Then, just when things were looking very serious for Frank, the report on the Riddles' bodies came back and changed everything. The police had never read an odder report. A team of doctors had examined the bodies and had concluded that none of the Riddles had been poisoned, stabbed, shot, strangles, suffocated, or (as far as they could tell) harmed at all. In fact (the report continued, in a tone of unmistakable bewilderment), the Riddles all appeared to be in perfect health – apart from the fact that they were all dead. The doctors did note (as though determined to find something wrong with the bodies) that each of the Riddles had a look of terror upon his or her face – but as the frustrated police said, whoever heard of three people being frightened to death? As there was no proof that the Riddles had been murdered at all, the police were forced to let Frank go. The Riddles were buried in the Little Hangleton churchyard, and their graves remained objects of curiosity for a while. To everyone's surprise, and amid a cloud of suspicion, Frank Bryce returned to his cottage on the grounds of the Riddle House. â€Å"As far as I'm concerned, he killed them, and I don't care what the police say,† said Dot in the Hanged Man. â€Å"And if he had any decency, he'd leave here, knowing as how we knows he did it.† But Frank did not leave. He stayed to tend the garden for the next family who lived in the Riddle House, and then the next – for neither family stayed long. Perhaps it was partly because of Frank that the new owners said there was a nasty feeling about the place, which, in the absence of inhabitants, started to fall into disrepair. The wealthy man who owned the Riddle House these days neither lived there nor put it to any use; they said in the village that he kept it for â€Å"tax reasons,† though nobody was very clear what these might be. The wealthy owner continued to pay Frank to do the gardening, however. Frank was nearing his seventy-seventh birthday now, very deaf, his bad leg stiffer than ever, but could be seen pottering around the flower beds in fine weather, even though the weeds were starting to creep up on him, try as he might to suppress them. Weeds were not the only things Frank had to contend with either. Boys from the village made a habit of throwing stones through the windows of the Riddle House. They rode their bicycles over the lawns Frank worked so hard to keep smooth. Once or twice, they broke into the old house for a dare. They knew that old Frank's devotion to the house and the grounds amounted almost to an obsession, and it amused them to see him limping across the garden, brandishing his stick and yelling croakily at them. Frank, for his part, believed the boys tormented him because they, like their parents and grandparents, though him a murderer. So when Frank awoke one night in August and saw something very odd up at the old house, he merely assumed that the boys had gone one step further in their attempts to punish him. It was Frank's bad leg that woke him; it was paining him worse than ever in his old age. He got up and limped downstairs into the kitchen with the idea of refilling his hot-water bottle to ease the stiffness in his knee. Standing at the sink, filling the kettle, he looked up at the Riddle House and saw lights glimmering in its upper windows. Frank knew at once what was going on. The boys had broken into the house again, and judging by the flickering quality of the light, they had started a fire. Frank had no telephone, in any case, he had deeply mistrusted the police ever since they had taken him in for questioning about the Riddles' deaths. He put down the kettle at once, hurried back upstairs as fast as his bad leg would allow, and was soon back in his kitchen, fully dressed and removing a rusty old key from its hook by the door. He picked up his walking stick, which was propped against the wall, and set off into the night. The front door of the Riddle House bore no sign of being forced, nor did any of the windows. Frank limped around to the back of the house until he reached a door almost completely hidden by ivy, took out the old key, put it into the lock, and opened the door noiselessly. He let himself into the cavernous kitchen. Frank had not entered it for many years; nevertheless, although it was very dark, he remembered where the door into the hall was, and he groped his way towards it, his nostrils full of the smell of decay, ears pricked for any sound of footsteps or voices from overhead. He reached the hall, which was a little lighter owing to the large mullioned windows on either side of the front door, and started to climb the stairs, blessing the dust that lay thick upon the stone, because it muffled the sound of his feet and stick. On the landing, Frank turned right, and saw at once where the intruders were: At the every end of the passage a door stood ajar, and a flickering light shone through the gap, casting a long sliver of gold across the black floor. Frank edged closer and closer, he was able to see a narrow slice of the room beyond. The fire, he now saw, had been lit in the grate. This surprised him. Then he stopped moving and listened intently, for a man's voice spoke within the room; it sounded timid and fearful. â€Å"There is a little more in the bottle, My Lord, if you are still hungry.† â€Å"Later,† said a second voice. This too belonged to a man – but it was strangely high-pitched, and cold as a sudden blast of icy wind. Something about that voice made the sparse hairs on the back of Frank's neck stand up. â€Å"Move me closer to the fire, Wormtail.† Frank turned his right ear toward the door, the better to hear. There came the clink of a bottle being put down upon some hard surface, and then the dull scraping noise of a heavy chair being dragged across the floor. Frank caught a glimpse of a small man, his back to the door, pushing the chair into place. He was wearing a long black cloak, and there was a bald patch at the back of his head. Then he went out of sight again. â€Å"Where is Nagini?† said the cold voice. â€Å"I – I don't know, My Lord,† said the first voice nervously. â€Å"She set out to explore the house, I think†¦Ã¢â‚¬  â€Å"You will milk her before we retire, Wormtail,† said the second voice. â€Å"I will need feeding in the night. The journey has tired me greatly.† Brow furrowed, Frank inclined his good ear still closer to the door, listening very hard. There was a pause, and then the man called Wormtail spoke again. â€Å"My Lord, may I ask how long we are going to stay here?† â€Å"A week,† said the cold voice. â€Å"Perhaps longer. The place is moderately comfortable, and the plan cannot proceed yet. It would be foolish to act before the Quidditch World Cup is over.† Frank inserted a gnarled finger into his ear and rotated it. Owing, no doubt, to a buildup of earwax, he had heard the word â€Å"Quidditch,† which was not a word at all. â€Å"The – the Quidditch World Cup, My Lord?† said Wormtail. (Frank dug his finger still more vigorously into his ear.) â€Å"Forgive me, but – I do not understand – why should we wait until the World Cup is over?† â€Å"Because, fool, at this very moment wizards are pouring into the country from all over the world, and every meddler from the Ministry of Magic will be on duty, on the watch for signs of unusual activity, checking and double-checking identities. They will be obsessed with security, lest the Muggles notice anything. So we wait.† Frank stopped trying to clear out his ear. He had distinctly heard the words â€Å"Ministry of Magic,† â€Å"wizards,† and â€Å"Muggles.† Plainly, each of these expressions meant something secret, and Frank could think of only two sorts of people who would speak in code: spies and criminals. Frank tightened his hold on his walking stick once more, and listened more closely still. â€Å"Your Lordship is still determined, then?† Wormtail said quietly. â€Å"Certainly I am determined, Wormtail.† There was a note of menace in the cold voice now. A slight pause followed – and the Wormtail spoke, the words tumbling from him in a rush, as though he was forcing himself to say this before he lost his nerve. â€Å"It could be done without Harry Potter, My Lord.† Another pause, more protracted, and then – â€Å"Without Harry Potter?† breathed the second voice softly. â€Å"I see†¦Ã¢â‚¬  â€Å"My Lord, I do not say this out of concern for the boy!† said Wormtail, his voice rising squeakily. â€Å"The boy is nothing to me, nothing at all! It is merely that if we were to use another witch or wizard – any wizard – the thing could be done so much more quickly! If you allowed me to leave you for a short while – you know that I can disguise myself most effectively – I could be back here in as little as two days with a suitable person -â€Å" â€Å"I could use another wizard,† said the cold voice softly, â€Å"that is true†¦Ã¢â‚¬  â€Å"My Lord, it makes sense,† said Wormtail, sounding thoroughly relieved now. â€Å"Laying hands on Harry Potter would be so difficult, he is so well protected -â€Å" â€Å"And so you volunteer to go and fetch me a substitute? I wonder†¦perhaps the task of nursing me has become wearisome for you, Wormtail? Could this suggestion of abandoning the plan be nothing more than an attempt to desert me?† â€Å"My Lord! I – I have no wish to leave you, none at all -â€Å" â€Å"Do not lie to me!† hissed the second voice. â€Å"I can always tell, Wormtail! You are regretting that you ever returned to me. I revolt you. I see you flinch when you look at me, feel you shudder when you touch me†¦Ã¢â‚¬  â€Å"No! My devotion to Your Lordship -â€Å" â€Å"Your devotion is nothing more than cowardice. You would not be here if you had anywhere else to go. How am I to survive without you, when I need feeding every few hours? Who is to milk Nagini?† â€Å"But you seem so much stronger, My Lord -â€Å" â€Å"Liar,† breathed the second voice. â€Å"I am no stronger, and a few days alone would be enough to rob me of the little health I have regained under your clumsy care. Silence!† Wormtail, who had been sputtering incoherently, fell silent at once. For a few seconds, Frank could hear nothing but the fire crackling. The second man spoke once more, in a whisper that was almost a hiss. â€Å"I have my reasons for using the boy, as I have already explained to you, and I will use no other. I have waited thirteen years. A few more months will make no difference. As for the protection surrounding the boy, I believe my plan will be effective. All that is needed is a little courage from you, Wormtail – courage you will find, unless you wish to feel the full extent of Lord Voldermort's wrath -â€Å" â€Å"My Lord, I must speak!† said Wormtail, panic in his voice now. â€Å"All through our journey I have gone over the plan in my head – My Lord, Bertha Jorkin's disappearance will not go unnoticed for long, and if we proceed, if I murder -â€Å" â€Å"If?† whispered the second voice. â€Å"If? If you follow the plan, Wormtail, the Ministry need never know that anyone else has died. You will do it quietly and without fuss; I only wish that I could do it myself, but in my present condition†¦Come, Wormtail, one more death and our path to Harry Potter is clear. I am not asking you to do it alone. By that time, my faithful servant will have rejoined us -â€Å" â€Å"I am a faithful servant,† said Wormtail, the merest trace of sullenness in his voice. â€Å"Wormtail, I need somebody with brains, somebody whose loyalty has never wavered, and you, unfortunately, fulfill neither requirement.† â€Å"I found you,† said Wormtail, and there was definitely a sulky edge to his voice now. â€Å"I was the one who found you. I brought you Bertha Jorkins.† â€Å"That is true,† said the second man, sounding amused. â€Å"A stroke of brilliance I would not have thought possible from you, Wormtail – though, if truth be told, you were not aware how useful she would be when you caught her, were you?† â€Å"I – I thought she might be useful, My Lord -â€Å" â€Å"Liar,† said the second voice again, the cruel amusement more pronounced than ever. â€Å"However, I do not deny that her information was invaluable. Without it, I could never have formed our plan, and for that, you will have your reward, Wormtail. I will allow you to perform an essential task for me, one that many of my followers would give their right hands to perform†¦Ã¢â‚¬  â€Å"R-really, My Lord? What -?† Wormtail sounded terrified again. â€Å"Ah, Wormtail, you don't want me to spoil the surprise? Your part will come at the very end†¦but I promise you, you will have the honor of being just as useful as Bertha Jorkins.† â€Å"You†¦you†¦Ã¢â‚¬  Wormtail's voice suddenly sounded hoarse, as though his mouth had gone very dry. â€Å"You†¦are going†¦to kill me too?† â€Å"Wormtail, Wormtail,† said the cold voice silkily, â€Å"why would I kill you? I killed Bertha because I had to. She was fit for nothing after my questioning, quite useless. In any case, awkward questions would have been asked if she had gone back to the Ministry with the news that she had met you on her holidays. Wizards who are supposed to be dead would do well not to run into Ministry of Magic witches at wayside inns†¦Ã¢â‚¬  Wormtail muttered something so quietly that Frank could not hear it, but it made the second man laugh – an entirely mirthless laugh, cold as his speech. â€Å"We could have modified her memory? But Memory Charms can be broken by a powerful wizard, as I proved when I questioned her. It would be an insult to her memory not to use the information I extracted from her, Wormtail.† Out in the corridor, Frank suddenly became aware that the hand gripping his walking stick was slippery with sweat. The man with the cold voice had killed a woman. He was talking about it without any kind of remorse – with amusement. He was dangerous – a madman. And he was planning more murders – this boy, Harry Potter, whoever he was – was in danger – Frank knew what he must do. Now, if ever, was the time to go to the police. He would creep out of the house and head straight for the telephone box in the village†¦but the cold voice was speaking again, and Frank remained where he was, frozen to the spot, listening with all his might. â€Å"One more murder†¦my faithful servant at Hogwarts†¦Harry Potter is as good as mine, Wormtail. It is decided. There will be no more argument. But quiet†¦I think I hear Nagini†¦Ã¢â‚¬  And the second man's voice changed. He started making noises such as Frank had never heard before; he was hissing and spitting without drawing breath. Frank thought he must be having some sort of fit or seizure. And then Frank heard movement behind him in the dark passageway. He turned to look, and found himself paralyzed with fright. Something was slithering toward him along the dark corridor floor, and as it drew nearer to the sliver of firelight, he realized with a thrill of terror that it was a gigantic snake, at least twelve feet long. Horrified, transfixed, Frank stared as its undulating body cut a wide, curving track through the thick dust on the floor, coming closer and closer – What was he to do? The only means of escape was into the room where the two men sat plotting murder, yet if he stayed where he was the snake would surely kill him – But before he had made his decision, the snake was level with him, and then, incredibly, miraculously, it was passing; it was following the spitting, hissing noises made by the cold voice beyond the door, and in seconds, the tip of its diamond-patterned tail had vanished through the gap. There was sweat on Frank's forehead now, and the hand on the walking stick was trembling. Inside the room, the cold voice was continuing to hiss, and Frank was visited by a strange idea, an impossible idea†¦This man could talk to snakes. Frank didn't understand what was going on. He wanted more than anything to be back in his bed with his hot-water bottle. The problem was that his legs didn't seem to want to move. As he stood there shaking and trying to master himself, the cold voice switched abruptly to English again. â€Å"Nagini has interesting news, Wormtail,† it said. â€Å"In-indeed, My Lord?† said Wormtail. â€Å"Indeed, yes,† said the voice, â€Å"According to Nagini, there is an old Muggle standing right outside this room, listening to every word we say.† Frank didn't have a chance to hide himself. There were footsteps and then the door of the room was flung wide open. A short, balding man with graying hair, a pointed nose, and small, watery eyes stood before Frank, a mixture of fear and alarm in his face. â€Å"Invite him inside, Wormtail. Where are your manners?† The cold voice was coming from the ancient armchair before the fire, but Frank couldn't see the speaker. the snake, on the other hand, was curled up on the rotting hearth rug, like some horrible travesty of a pet dog. Wormtail beckoned Frank into the room. Though still deeply shaken, Frank took a firmer grip on his walking stick and limped over the threshold. The fire was the only source of light in the room; it cast long, spidery shadows upon the walls. Frank stared at the back of the armchair; the man inside it seemed to be even smaller than his servant, for Frank couldn't even see the back of his head. â€Å"You heard everything, Muggle?† said the cold voice. â€Å"What's that you're calling me?† said Frank defiantly, for now that he was inside the room, now that the time had come for some sort of action, he felt braver; it had always been so in the war. â€Å"I am calling you a Muggle,† said the voice coolly. â€Å"It means that you are not a wizard.† â€Å"I don't know what you mean by wizard,† said Frank, his voice growing steadier. â€Å"All I know is I've heard enough to interest the police tonight, I have. You've done murder and you're planning more! And I'll tell you this too,† he added, on a sudden inspiration, â€Å"my wife knows I'm up here, and if I don't come back -â€Å" â€Å"You have no wife,† said the cold voice, very quietly. â€Å"Nobody knows you are here. You told nobody that you were coming. Do not lie to Lord Voldemort, Muggle, for he knows†¦he always knows†¦Ã¢â‚¬  â€Å"Is that right?† said Frank roughly. â€Å"Lord, is it? Well, I don't think much of your manners, My Lord. Turn 'round and face me like a man, why don't you?† â€Å"But I am not a man, Muggle,† said the cold voice, barely audible now over the crackling of the flames. â€Å"I am much, much more than a man. However†¦why not? I will face you†¦Wormtail, come turn my chair around.† The servant gave a whimper. â€Å"You heard me, Wormtail.† Slowly, with his face screwed up, as though he would rather have done anything than approach his master and the hearth rug where the snake lay, the small man walked forward and began to turn the chair. The snake lifted its ugly triangular head and hissed slightly as the legs of the chair snagged on its rug. And then the chair was facing Frank, and he saw what was sitting in it. His walking stick fell to the floor with a clatter. He opened his mouth and let out a scream. He was screaming so loudly that he never heard the words the thing in the chair spoke as it raised a wand. There was a flash of green light, a rushing sound, and Frank Bryce crumpled. He was dead before he hit the floor. Two hundred miles away, the boy called Harry Potter woke with a start.

Bacterial Staining

Certain stains can also be used to identify internal structures of the cell, which would otherwise be unseen. Further, in order to use the oil immersion objective of the microscope and thereby obtain the iratest degree of magnification, it is convenient to use stained preparations rather than wet mounts. L Although bacteria do not appear greatly different from their surroundings, they differ chemically. It is this chemical difference that enables us to distinguish bacteria by staining, the stain or dye readily reacting with the bacterial cell but not with the background. Preparation of Smear Before Staining (figure 1) 1. Prepare a clean slide. Put a proper label. 2. Heat your loop to sterilize it. 3. For solid media: 0 Using a sterile inoculating loop, place a 1 or 2 loophole of distilled water on the enter of the slide. 0 Scrape a small amount of the culture off the slant. 0 Smear on the center of the slide, with the distilled water, the scraped material. For liquid media: 0 Make a smear from the broth. You don't have to add water as the bacteria are already suspended in water. Use 2 or 3 loophole of the culture. 0 Spread the culture on the center of the slide. 4. Reheat the loop to clean it. 5. Let the smear dry. – air dry – heat fix- passing the slide through a flame 2 or 3 times Figure 1. Preparation of smear. Classification of Stain Based on Functions 1. Simple Staining Method – In this method one aniline dye is used to stain the organism to be studied. 2. Differential Staining Method – Under this type of classification, the staining method employed divides the microorganism into groups.The Gram's Staining Method and Acid-Fast (Zilch-Nielsen Method) fall under this category. 3. Selective or Special Staining Method – Under this category, parts or portion of the cell are stained differently from the rest of the cell. 4. Indirect Staining Method – Indirect stains are also relief stains because it is the background whi ch takes up the taint, not the organism and the organism are only seem by contrast. 2 Examples of Staining Simple Staining Methods Use only 1 stain. Use to determine cell morphology, size and arrangement.Procedure: a. Make a smear. B. Staining: 1. Place the slide on a staining rack. 2. Flood the smear with several drops of the dye, allow it to remain for the following intervals: – Carbon-fuchsia – 15 to 30 seconds – Methyl Blue – 2 to 5 minutes – Crystal violet – 30 to 45 seconds 3. Carefully wash the excess stain off with distilled water from a wash bottle. Let the water run down the tilted slide. 4. Gently blot the smear with a paper towel or absorbent paper and let it dry. C. View the prepared slide under the microscope. Results and reaction: Reaction / Results Principle Samples of Bacteria All bacteria in smear takes stain Simple stains use basic dyes All types of and appears in color of stain which are positively bacteria. Charged. Thes e positive dyes 0 Shape: interact with the slightly 0 Spherical – coca negatively charged bacterial 0 Rod – bacilli cell wall thus lending the color 0 Arrangement : of the dye to the cell wall. 0 Coca in clusters – staphylococci 0 Coca in chains – thyrotrophic 2. A.Gram's Stains most common technique the gram stain is valid only when performed on young (less than 24 hours old) cultures of bacteria Procedure:l b. Gram staining: Steps Purpose 1. Use a clothespin or slide rack to hold the slide. 3 2. Cover the smear with crystal violet and leave for 30 seconds. 3. Wash the slide carefully with distilled water from a wash bottle. O do not squirt the water directly Primary stain – all bacteria are stained purple. Onto the smear 4. Without drying, cover the smear with Gram's iodine for 30 seconds. 5.Without washing, decolonize with 95% ethyl alcohol. Let the alcohol run through the smear until no large amount of purple wash out. O do not over decolonize 6. Immediately wash with distilled water. 7. Add seafaring for 30 seconds. Mordant – this intensifies the ionic bond between the primary stain and the Primary stain is washed out of some bacteria, while others are unaffected. Secondary stain or countersink – stains the decolonize bacteria red. 8. Wash with distilled water and blot the slide with a paper towel or absorbent paper. Let dry. C. Examine under the microscope. 9.Results: Reactions / Result Gram cell wall are thick and chemically costive (+) simple, composed mainly of 0 purple protein and cross-linked colored minicomputers – alcohol causes dehydration and shrinkage of the gram+ cell wall – reducing the loss of substances such as crystal violet Aggregative (-) 0 pink wall is a thin, complex, multilayered structure containing protein, minicomputers and lipids – when treated with alcohol, the lipid dissolves and the primary stain is wash out Samples of Bacteria Gram positive coca in clusters (figure AAA): Staphylococci species Gram positive bacilli (figure ad): Colostomies species Crematoriums Bacillus anthracicGram negative coca in chains: – Streptococci Gram negative coca (figure 4 – Engineers species Gram negative bacilli (figure c): – Escherichia coli – Kielbasa pneumonia b d Figure 2. Different observations in Gram's Staining. (a)gram+ coca in clusters (b)gram + coca in chains (c)gram- bacilli (d)gram+ bacilli (e)gram- coca. (f)gram stain mixed 5 B. Acid – Fast Stain (Zilch – Nielsen Stain)l ,5 0 to stain Mycobacterium species especially M. Tuberculosis – Contain large amount of fatty waxes (mycology acid) within their cell wall resists staining by ordinary methods 0 Procedure: 1. Flood smear with Carbon Fuchsia Carbon Fuchsia is a lipid soluble, stain. Heinlein compound, which is able to penetrate the cell wall. 2. Cover flooded smear with filter paper 3. Steam for 10 minutes. Add more Carbon Fuchsia stain as needed . 4. Cool slide. 5. Rinse with distilled water. 6. Flood slide with acid alcohol (leave 15 The waxy cell wall then prevents the seconds). The acid alcohol contains stain from being removed by the acid 3% HCI and 95% ethanol or H2O alcohol (decolonize) once it has SIS. Penetrated the cell wall. The acid alcohol decolonize will remove the stain from all other cells. . Tilt slide 45 degrees over the sink and add acid alcohol drop wise (drop by drop) until the red color stops streaming from the smear. 8. Rinse with distilled water

Visit to Сourt

?I have got a chance to visit court on 2, January 2014 with my group members Biagio Mauri for our law assignment. This is the first time of my life visiting to court and we went to magistrate court around 1pm. That court is located in 363 George Street Brisbane QLD 4001, opening and closing hours is 8:30-4:30. When we arrived infront of the court I feel alittle nervous because I have never been to any court. My group members asked me to turn off all of my electronic devices.In entrance there are some sign of no food /drink and turn off electronic devices. I stepped into the entrance and the first thing I see is security woman with desk in front of the court. She was friendly and asked us to step back to x-ray machines which is use in airport or any other security check . Then she asked us to come one by one, so that my group member would go first and I was waiting him outside of the entrance. After she checked my group member’s bag with metal detectors as she asked me to come in and she did the same way.After security check, we ahead to information counter and we saw some people were asking about the court room that related to their cases. So we qued for a little while and we got reach our trun. My group member requested to receptionist that we are student doing law assignment and which room we need to go. Then she said room 36 is just started and pointed us to get there. I felt so excited to get into the room as I saw auto double door to get into the room. I became noticed everyone is quiet and we took chair in last place.Although I knew that I need to be quiet but my group member remind me to be quiet. After we sat down,I started to discover the circumstances. There were few people sitting with us. The name of the judge was Carmody T and he looked greate on his formal black suit with the white collar. His place was the highest positon of the room and he was sitting. At the second positon, I saw a typist women and she was typing about the case. At the l ast positon I saw one lawyer and one policeman who standing were had conversation with the judge.I saw around 4 or 5 police infront of the 2 small glass room that they made with protective glass for protect people from the accuse abuse I guess. After I did sightseeing ,the first accuse was already got judgement . So I started focus on second accuse case and listened carefully. Before the accuse come, the lawer talked about the case. That case was nature of crime about theft case, the police woman took the accuse man from the door behind that glass room. The judge asked that accuse man that he feel gulity or not. He said he felt gulity so that judge continue ask about what he did steal.He admited that he stolen $4658 cash from the high school. Finally the judge made the decision that he needed to give fine $2000 and sent to prison for 6 months. In conclusion, I got a lot of knowledges about the court by visiting magistrate court in brisbane Australia. I experienced about how the judg e and lawyer analysis the case and the court procedure. When I left that room I did bow my head down as other people did. I realized how court is important for a countrty, if court does not exist we cannot exactly know what is justice.

Customer Relationship Marketing Essays - Marketing, Free Essays

Customer Relationship Marketing Essays - Marketing, Free Essays Customer Relationship Marketing Literature Review The Evolving Sales and Marketing Landscape Marketing and business development professionals are confronting a rapidly different and changing business landscape. The traditional business model that was once the standard is now being transformed due to technology drivers that make advanced marketing and sales capabilities possible. The business model of yesterday supported mass marketing, mass production, and standardized cookie-cutter products and services. Enterprises will have fall behind the competition if they continue to rely and operate on this substandard model. Today, companies are re-engineering their operations and investing in enhanced IT infrastructures, which enable them to provide customized, personalized, information-rich products and services. The new objective for marketers and business developers involves understanding the needs of their clients and the markets that they serve. This new focus on providing customer value is redefining business processes. Professionals, who understand and anticipate this shift, are positioning themselves ahead of the competition. The Impact of Technology Enabled Business Processes There is no denying the effect that technology has had on the way we conduct business. In recent years, technology has begun to play a significantly larger role in all aspects of business, including sales and marketing. Business processes must be re-engineered to incorporate a pro-active strategy for using information and IT to build a competitive advantage over other organizations. In many instances, the functionality provided by sales and marketing technology only automates current processes. Therefore, it is imperative that processes are clearly defined and well proven. Automating a poorly understood or followed process usually results in failure. Technology must be used as an enabler to support an already sound sales and marketing strategy. Beyond automating sales and marketing capabilities, technology is now designed to provide professionals with a wealth of information about their companys clients and the markets that they serve. Companies are now able to collect data about their customers that when analyzed and utilized properly, can result in a competitive advantage. Online shopping is a prime example. If a customer makes a purchase, then the transaction is completed and revenue is generated. However, if a sales transaction is not made, the company still collects the shoppers behavioral data and stores it in a marketing database. The company then has the opportunity to segment and analyze this data in order to learn more about that particular customer shopping habits. An analysis can then be performed to determine why this person did not make a purchase as well as what can be done differently to persuade this person to buy their product. The company can now position the four Ps of marketing: product, price, promotion, and place to better serve this customer. This process the company has taken unlitmately succeeds in developing a relationship with the customer. The company now knows more about what the customers preferences are and how to offer them. Consequently, the customer has a vested interest in the relationship as well, and is more likely to conduct business with that company in the future. The Shift to Relationship Marketing Though many of today's marketing practices have evolved due to increasing technology, the marketing environment still centers around the four Ps of marketing described above. However, in order to remain competitive, companies must create a unique set of those four Ps which appeal to each individual customer and sets them apart from their competitors. Target marketing is no longer an efficient use of marketing efforts because a great deal of

Totalitarian Dictators essays

Totalitarian Dictators essays The first things that come to mind when the names Stalin, Mussolini and Hitler are mentioned are the cruel, tyrannical, inhumane acts they committed against their own people, and people of other nationalities. But, what one fails to see is the public works they sponsored, the ways they modernized their countries, and other beneficial causes they supported. Without these dictators the countries of Russia, Italy and Germany would have all been affected in very distinct ways; and although their rules were mainly counterproductive, the actions they took to improve the lives of their people would in some cases bring them out of a depression, and in other cases modernize them, in times when their countries called for it most. Joseph Stalins devotion to the non-dependency of the much-rivaled capitalist westerners pushed him and the rest of communist Russia to strive for modernization and self-sufficiency. His accomplishments consist of industrializing the U.S.S.R. in a time when their position as a world power was questioned. Stalins Five-Year plan was devised to prepare the U.S.S.R. in defending itself from inevitable war with the capitalist countries of the west. Although many lives were sacrificed in this process his goal was ultimately achieved. If not for his timing the U.S.S.R. would clearly not have stood a chance against its hated rival Nazi Germany in World War II. Stalin, however cruel he was, enforced a crucial part of Russian modernization, that if overlooked would have led them to a humiliating defeat in the Second World War. Benito Mussolini was very effective at raising the spirits of Italians, boosting their poor economy, and strengthening their week military, as well as supporting public projects, and education. Mussolini was able to accomplish all of these remarkable tasks because his fore-most goal was to make Italy a great power of Europe. He was able to support public projects like the revitalization of ...